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Part:BBa_K1351037:Design

Designed by: Nikolai Peschek   Group: iGEM14_LMU-Munich   (2014-10-06)

P2-promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part has been artificially synthesized with mutated illegal restriction sites. These sites have been mutated with regard to the codon usage of Bacillus subtilis.

Source

This part was generated by amplification from artificially synthesized DNA with the primers listed below, followed by digestion with EcoRI and PstI and ligation into pSB1C3.

P2_fwd: GATCGAATTCGCGGCCGCTTCTAGAGGCATTTATTTTCCAATTTTTCTTAACTAG

P2_rev: GATCACTAGTACCTCACTGTTATTATACGATTTAGTAC

Annealing sequences are written in bold.

References

Reynolds, J. and S. Wigneshweraraj (2011). "Molecular Insights into the Control of Transcription Initiation at the Staphylococcus aureus agr operon." Journal of Molecular Biology 412(5): 862-881.